CYTOTOXICITY SCREENING MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY FOR NANOPARTICLE BIOMARKER DETECTION by

Toxicology is a broad topic that aims to assess the risk of chemicals on living organisms. It has been acknowledged that everything is toxic to a living organism. What distinguishes a toxin from a remedy depends on the dose 1 . Presently, there are toxicity assays that are implemented for human risk assessment studies such as, 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2Htetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) assay; however, it has been shown that some toxins interfere with these assays. Carbon nanodots, an unknown toxicant has shown to interfere with the LDH assay. Therefore proper methods and techniques must be devised for the analysis of these unknown toxicants to enable the reliable risk assessment for humans.